GSM2092845: LNCaP NOV AR ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
AR (santa cruz, sc-815X, lot K0713)
Sequenced DNA Library
Chromatin in nuclear fraction were sheared to 200-500bp using an ultrasonic sonicator (Misonix), Lysates were clarified from sonicated nuclei and protein-DNA complexes were immunoprecipitated using the indicated antisera. DNA was then uncrosslinked from protein, purified and sequenced using Illumina Genome Analyzers followig protocols recommended by the manufacturer. Libraries were prepared according to Illumina's instructions.