Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

MOLT-4

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

T-ALL

cell line

MOLT4

antibody

RNA pol II CTD phospho Ser2

antibody manufacturer

Active Motif

antibody catalog number

3E10

drug treatment

DMSO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Standard DNA extraction from MOLT4 cell lines using cell lysis Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 60% amplitude) on ice library prep with Rubicon TruePlex kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

74042352

Reads aligned (%)

57.1

Duplicates removed (%)

6.9

Number of peaks

5163 (qval < 1E-05)

hg19

Number of total reads

74042352

Reads aligned (%)

56.8

Duplicates removed (%)

7.4

Number of peaks

4848 (qval < 1E-05)