Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
MOLT-4
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
T-ALL
cell line
MOLT4
antibody
BRD4
antibody manufacturer
Bethyl
antibody catalog number
A301-985A100
drug treatment
JQ1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard DNA extraction from MOLT4 cell lines using cell lysis Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 60% amplitude) on ice library prep with Rubicon TruePlex kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
67554976
Reads aligned (%)
71.7
Duplicates removed (%)
9.5
Number of peaks
25817 (qval < 1E-05)

hg38

Number of total reads
67554976
Reads aligned (%)
73.8
Duplicates removed (%)
7.8
Number of peaks
27349 (qval < 1E-05)

Base call quality data from DBCLS SRA