Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
EZH2_WT_H3K27_BCELLS_Input
strain background
C57BL/6
genotype/variation
wild type
tissue/cell type
Spleen
cell type
CD19+ splenic B cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
B cells were isolated from animals' spleen. Briefly, spleens were made into single cell suspension, stained with CD19-biotin, washed, stained with antibiotin magnetic beads and separated on an Automacs magnetic separator. Illumina Truseq ChIPseq kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
36043180
Reads aligned (%)
98.5
Duplicates removed (%)
11.5
Number of peaks
529 (qval < 1E-05)

mm9

Number of total reads
36043180
Reads aligned (%)
98.2
Duplicates removed (%)
11.5
Number of peaks
571 (qval < 1E-05)

Base call quality data from DBCLS SRA