Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Trophoblasts
MeSH Description
Trophoblasts with multinuclear cytoplasm and undefined cellular structures. Syncytiotrophoblasts secrete proteolytic enzymes that erode the ENDOMETRIUM for EMBRYO IMPLANTATION. They also produce steroid, peptide, and protein hormones (PLACENTAL HORMONES).

Attributes by original data submitter

Sample

source_name
mouse embryo
development stage
trophoblast
chip antibody
none
overexpression
B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
14649376
Reads aligned (%)
95.2
Duplicates removed (%)
18.9
Number of peaks
165 (qval < 1E-05)

mm9

Number of total reads
14649376
Reads aligned (%)
95.1
Duplicates removed (%)
18.9
Number of peaks
151 (qval < 1E-05)

Base call quality data from DBCLS SRA