Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Blastocyst Inner Cell Mass

MeSH Description

The cluster of cells inside a blastocyst. These cells give rise to the embryonic disc and eventual embryo proper. They are pluripotent EMBRYONIC STEM CELLS capable of yielding many but not all cell types in a developing organism.

Sample

source_name

mouse embryo

development stage

inner cell mass

chip antibody

none

overexpression

B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

10726692

Reads aligned (%)

93.3

Duplicates removed (%)

19.4

Number of peaks

169 (qval < 1E-05)

mm9

Number of total reads

10726692

Reads aligned (%)

93.2

Duplicates removed (%)

19.4

Number of peaks

141 (qval < 1E-05)