Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
4-cell stage
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryo
development stage
8-cell embryo
chip antibody
H3K27me3(Diagnode, pAb-069-050)
overexpression
B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
13436010
Reads aligned (%)
92.9
Duplicates removed (%)
47.1
Number of peaks
60 (qval < 1E-05)

mm9

Number of total reads
13436010
Reads aligned (%)
92.8
Duplicates removed (%)
47.1
Number of peaks
53 (qval < 1E-05)

Base call quality data from DBCLS SRA