Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

4-cell stage

NA

NA

Sample

source_name

mouse embryo

development stage

4-cell embryo

chip antibody

H3K4me3(Cell signaling Technology, #9727)

overexpression

B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

18071744

Reads aligned (%)

99.0

Duplicates removed (%)

11.3

Number of peaks

486 (qval < 1E-05)

mm9

Number of total reads

18071744

Reads aligned (%)

98.9

Duplicates removed (%)

11.4

Number of peaks

479 (qval < 1E-05)