Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Oocytes
MeSH Description
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Attributes by original data submitter

Sample

source_name
MII oocyte
development stage
MII Oocyte
chip antibody
none
overexpression
B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
17025445
Reads aligned (%)
99.2
Duplicates removed (%)
13.1
Number of peaks
175 (qval < 1E-05)

mm9

Number of total reads
17025445
Reads aligned (%)
99.1
Duplicates removed (%)
13.2
Number of peaks
140 (qval < 1E-05)

Base call quality data from DBCLS SRA