Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Prostate

Cell type

C4-2

Tissue

Prostate

Cell Type

Epithelial

Disease

Prostate Cancer

Sample

source_name

Human prostate cancer cell line C42B

cell line

C42B

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq, RNA was extracted using Trizol reagent (15596-026) (Life technologies, Carlsbad, CA) following its protocol. For ChIP-seq, cells were crosslinked using 1% formaldehyde andl lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. RNA-seq libraries were made using KAPA Stranded mRNA-Seq Kit with KAPA mRNA Capture Beads (KK8420) (Kapa Biosystems, Woburn, MA). ChIP-seq: Libraries were barcoded (NEXTflex™ DNA Barcodes) (Bio Scientific, Austin, TX).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

50996550

Reads aligned (%)

98.7

Duplicates removed (%)

3.9

Number of peaks

1826 (qval < 1E-05)

hg19

Number of total reads

50996550

Reads aligned (%)

97.5

Duplicates removed (%)

6.0

Number of peaks

1537 (qval < 1E-05)