GSM2080680: ES input Ring1b; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESC input for Ring1b ChIP
cell type
ESC
strain
J1
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested and MEFs depleted from ESC cultures. ESCs and NSCs were crosslinked in 1% formaldehyde for 10 minutes at room temperature. ChIP was carried out as described in Illingworth et al. (2015). Briefly, cells were lysed and sonicated to an average fragment length of 250bp using a Qsonica. Chromatin was incubated with protein A dynabeads pre-bound with antibody overnight at 4C. Beads were washed and DNA eluted. Sequencing libraries were prepared as described in Bowman et al. (Bowman et al., 2013).