ChIP-Atlas: SRX1609549

Antigen

Cell type Class: Blood
Cell type: Erythroid Cells
MeSH Description: The series of cells in the red blood cell lineage at various stages of differentiation.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: A single cell suspension was made by gently dissociating the spleen of a phenyhydrazine treated mouse and passing it through a 70um filter. Ter119 selection was performed by staining the cells with phycoerythrin conjugated anti-ter119 antibodies (BD biosciences)). The cells were subsequently washed and then incubated with MACS anti-phycoerythrin beads. Cells were then separated using an auto-MACS (miltenyi biotec). Fragments double-crosslinked were immunoprecipitated with the antibody of interest (Med1). Libraries were prepared using the NEB Ultra library preparation kit according to manufacturer’s instructions. Samples were sequenced using 40 bp paired end reads on the NextSeq platform The strategy was to isolate at a genome-wide level the DNA regions bound by Med1. Because the binding is assumed to be indirect, stronger croslinking conditions were used (double crosslinking FA and DSG).