Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
C2C12 myotubes_input
cell line
C2C12
cell type
myoblast cell line
genotype/variation
wild type
treated with
100 nmol/L CDDO-Im for 3 h

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were fixed with 1% formaldehyde for 10 min at room temperature and subsequently quenched with 0.125-M glycine. ChIP was performed with anti-Nrf2 antibody (Cell Signaling Technology; D1Z9C).The antibody incubation were pereformed overnight at 4 °C. The DNA libraries were prepared from 2 ng of ChIP and input samples quantified with Qubit Fluorometer (Life Technologies), using Mondrian SP+ and Ovation SP Ultralow DR Multiplex System (TaKaRa).braries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
35962944
Reads aligned (%)
98.4
Duplicates removed (%)
32.3
Number of peaks
651 (qval < 1E-05)

mm9

Number of total reads
35962944
Reads aligned (%)
98.1
Duplicates removed (%)
32.3
Number of peaks
636 (qval < 1E-05)

Base call quality data from DBCLS SRA