Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP63

Cell type

Cell type Class
Epidermis
Cell type
Differentiated keratinocytes
NA
NA

Attributes by original data submitter

Sample

source_name
DK
cell type
Differentiated Keratinocytes
chip antibody
p63 (4A4)
chip antibody vendor
Santa Cruz Biotechnology
chip antibody lot number
C1512

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin preparation and processing was performed starting from pellets of 10e7 KP and DK cells and following the truChIP Chromatin Shering Kit standard protocol (PN520076, Covaris Inc.). Libraries were prepared according to Illumina's instructions accompanying the standard protocol of Illumina ChIP-seq DNA sample preparation kit (IP-102-1001, Illumina Inc.).

Sequencing Platform

instrument_model
Illumina MiSeq

hg19

Number of total reads
22347527
Reads aligned (%)
92.3
Duplicates removed (%)
3.8
Number of peaks
1686 (qval < 1E-05)

hg38

Number of total reads
22347527
Reads aligned (%)
94.3
Duplicates removed (%)
2.5
Number of peaks
1705 (qval < 1E-05)

Base call quality data from DBCLS SRA