GSM2071220: Erα Chipseq MCF7LUC INPUT; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 LUC cells
agent
Vehicle treatment
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed in MCF7_LUC and MCF7_Y537S cell lines. The cells were seeded at 10X10^6 cells/plate on 150cm dishes in DMEM lacking of phenol-red with 10% dextran-coated charcoal stripped FCS for 72 hours, then treated with ethanol/oestrogen (10nM) for 45 mins prior to fixation. Lysates were collected from sonicated nuclei extracts and subsequently were immuno-precipitated with Erα antibody (HC-20, santacruz). DNA was then extracted using phenol-chloroform and sodium chloride precipitation method. For Chip-seq, 10ng DNA from IP and input samples were used for library preparation. Libraries were prepared according to NEB (E7370S) multiplex library preparation protocol for DNA-seq preparation. Libraries were run on bioanalyzer for quality control. Finally, sequencing was performed on Ilumina Hiseq 2000 genome analyser (using 50b reads)