GSM2070586: Pooled (human+Drosophila) input control; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Gonad
Cell type
Tcam-2
NA
NA
Attributes by original data submitter
Sample
source_name
pooled input DNA of Romidepsin and solvent treated TCam-2 DNA, including Drosophila DNA spike-in control
cell line
Tcam-2
cell type
Seminoma-like cell line / Drosophila DNA (spike-in)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin-immunoprecipitation-followed-by-sequencing (ChIP-seq) analysis, cells of interest were fixed by addition of 1/10 Formaldehyde solution (11 % Formaldehyde, 0.1 M NaCl, 1 mM EDTA (pH 8), 50 mM HEPES (pH7.9)) to the culture media followed by 15 minutes of incubation at room temperature. Fixation was stoped by addition of 1/20 volume Glycine solution (2.5 M) and incubation for 5 minutes at room temperature. Cells were scraped and washed once in PBS-Igepal (0.5%) solution and once more in PBS-Igepal-PMSF (1mM) solution. Cells were pelleted by centrifugation and snap frozen on dry ice and stored at -80°C.