Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Muscle

Cell type

LHCN-M2

Tissue

skeletal muscle myoblast

Lineage

mesoderm

Description

skeletal myoblasts derived from satellite cells from the pectoralis major muscle of a 41 year old caucasian heart transplant donor, immortalized with lox-hygro-hTERT ("LH"), and Cdk4-neo ("CN"), Zhu et al. (2007) in Aging Cell, vol. 6, pp 515-523, newly promoted to tier 2: not in 2011 analysis.

Sample

source_name

immortalized human myoblast line LHCN-M2

treatment dose

250 ng/ml doxycycline

treatment time

6hrs

antibody

H3K4me3 (abcam, ab8580)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

TruSeq ChIP Sample Prep Kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

55299476

Reads aligned (%)

98.4

Duplicates removed (%)

45.3

Number of peaks

43008 (qval < 1E-05)

hg19

Number of total reads

55299476

Reads aligned (%)

98.2

Duplicates removed (%)

45.6

Number of peaks

42839 (qval < 1E-05)