Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

haematopoietic stem and progenitor cell (HSC)

strain

C57BL/6J

chip antibody

none

cell type

haematopoietic stem and progenitor cell (HSC)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed with 1x10^7 cells per experiment. The cells were collected and cross-linked for 15min in 1% paraformaldehyde, washed and lysed. Chromatin was sheared using a Bioruptor to create fragments of approximately 150 base pairs, incubated with about 2–5 μg antibody bound to 75 μl Dynabeads M-280 (Invitrogen) and rotated overnight at 4 °C, then washed and eluted. The eluted chromatin was reverse-cross-linked and column purified. ChIP samples were prepared for sequencing using Illumina TruSeq DNA Sample Preparation Kit according to the standard preparation protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 1000

mm10

Number of total reads

37857852

Reads aligned (%)

84.6

Duplicates removed (%)

13.6

Number of peaks

667 (qval < 1E-05)

mm9

Number of total reads

37857852

Reads aligned (%)

84.3

Duplicates removed (%)

13.6

Number of peaks

744 (qval < 1E-05)