GSM2066627: H3K27me3 HFFs input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
foreskin
cell type
foreskin fibroblast
treatment
control
time
control
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Histones and DNA were cross-linked by 1% formaldehyde and extracted using a Magna ChIP G kit (Millipore). The chromatin was then sonicated to an average DNA fragment length of 200-300 bp. Illumina sequencing libraries were generated according to the manufacturer’s instructions. Briefly, 5-50 ng of ChIP product was end repaired, a-tailed and ligated to Illumina single end adapters by using an NEB Next kit (New England Biolabs). Adaptor-ligated DNA with a size of 200-300 bp was purified by running a gel and enriched via 19 cycles of PCR amplification with Phusion High-Fidelity DNA Polymerase, followed by sequencing on an Illumina HiSeq 2000 system.