ChIP-Atlas: SRX1585633

Antigen

Cell type Class: Lung
Cell type: Lung
MeSH Description: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Two biological replicates of pooled E18.5 lungs (n=3) were crosslinked with 37% formaldehyde to a final concentration of 1%. ChIP was performed using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling, 9005S). Micrococcal nuclease-digested chromatin was further sonicated using Diagenode Bioruptor with 20 pulses of 15s on and 15s off at high power to yield sheared chromatin. Ten micrograms of chromatin was used per immunoprecipitation with 7 µg of FOXF1 antibody. The Rubicon ThruPlex DNA-Seq library preparation system was used to prepare ChIP-Seq libraries for sequencing on the Illumina HiSeq sequencing system.