Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 breast cancer cells, Tamoxifen resistant
cell line
MCF7T
chip antibody
ERα antibody (HC-20) (Santa Cruz, catalog# sc-543)
passage
<10

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. NEB ultra kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
24539889
Reads aligned (%)
75.8
Duplicates removed (%)
20.8
Number of peaks
13849 (qval < 1E-05)

hg38

Number of total reads
24539889
Reads aligned (%)
77.6
Duplicates removed (%)
19.4
Number of peaks
13300 (qval < 1E-05)

Base call quality data from DBCLS SRA