Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT116 with homozygous deletion of DNMT3b and DNMT1 (Rhee et al Nature 2002)
technique
ChIP-sequencing
starting molecule
chromatin
antibody/capture
anti-H3K27me3 (Millipore, 07-449)

Sequenced DNA Library

library_name
GSM955165: ChIP-seq_HCT116_DKO_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
17678636
Reads aligned (%)
98.5
Duplicates removed (%)
6.7
Number of peaks
127 (qval < 1E-05)

hg19

Number of total reads
17678636
Reads aligned (%)
98.0
Duplicates removed (%)
7.2
Number of peaks
134 (qval < 1E-05)

Base call quality data from DBCLS SRA