Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
Mouse embryonic fibroblasts
strain
C57BL/6
age
Embryos were isolated from the uteri at embryonic day 10.5.
antibody
none, Input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. The libraries were sequenced on the Illumina Hiseq 2500 as single-end 50 base reads following Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
37291695
Reads aligned (%)
96.2
Duplicates removed (%)
52.3
Number of peaks
32782 (qval < 1E-05)

mm9

Number of total reads
37291695
Reads aligned (%)
96.0
Duplicates removed (%)
52.4
Number of peaks
32702 (qval < 1E-05)

Base call quality data from DBCLS SRA