Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562_H3K4me1

cell line

K562

cell type

human erythroleukemic cell line

passages

14

chip antibody

H3K4me1 (Abcam ab8895, GR251663-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

112619742

Reads aligned (%)

99.6

Duplicates removed (%)

39.8

Number of peaks

108977 (qval < 1E-05)

hg19

Number of total reads

112619742

Reads aligned (%)

99.5

Duplicates removed (%)

40.1

Number of peaks

108640 (qval < 1E-05)