Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562_H3K27ac

cell line

K562

cell type

human erythroleukemic cell line

passages

14

chip antibody

H3K27ac (Abcam ab4729, GR254707-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

134989850

Reads aligned (%)

99.4

Duplicates removed (%)

40.0

Number of peaks

68642 (qval < 1E-05)

hg19

Number of total reads

134989850

Reads aligned (%)

99.3

Duplicates removed (%)

40.3

Number of peaks

68365 (qval < 1E-05)