Antigen

Antigen Class

Histone

Antigen

H3K122ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

46c_H3K122ac

cell line

46c (sox1-GFP)

cell type

mouse embryonic stem cells

passages

25-28

chip antibody

anti-H3K122ac (Tropberger et al 2013, PMID: 23415232)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

192966946

Reads aligned (%)

88.9

Duplicates removed (%)

68.7

Number of peaks

69214 (qval < 1E-05)

mm9

Number of total reads

192966946

Reads aligned (%)

88.8

Duplicates removed (%)

68.8

Number of peaks

69389 (qval < 1E-05)