Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
GHFT1-5
NA
NA

Attributes by original data submitter

Sample

source_name
pituitary cell line
cell line
GHFT1
passages
18-23
ChIP
input DNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and biotinylated PROP1-DNA complexes were isolated with streptavidin beads. Libraries were prepared according to Rubicon ThruPlex kit protocol (CAT. NO. R40012).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
35771114
Reads aligned (%)
97.4
Duplicates removed (%)
9.0
Number of peaks
2725 (qval < 1E-05)

mm9

Number of total reads
35771114
Reads aligned (%)
97.3
Duplicates removed (%)
9.1
Number of peaks
2900 (qval < 1E-05)

Base call quality data from DBCLS SRA