GSM2047027: H3K4me1 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived cardiac cells
NA
NA
Attributes by original data submitter
Sample
source_name
iPSC-derived cardiomyocytes
cell type
iPSC-derived cardiomyocytes
passages
20-30
iPSc source
individual ID: YRI-NA18852
antibody
Millipore cat#07-436 lot#2684917
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Histone-DNA or PolII-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions provided with the TrueSeq ChIP Sample Preparation Kit (Catalog #: IP-202-1012). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. Coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250-450 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.