Antigen

Antigen Class

TFs and others

Antigen

TP53

Cell type

Cell type Class

Epidermis

Cell type

Dermal fibroblast

NA

NA

Sample

source_name

foreskin HDF, Nutlin, IP

cell line

foreskin HDF

passage

p4

chip antibody

p53-DO1 (Santa Cruz, catalog# sc126, lot#K2613)

treatment

Nutlin-3a 10 μM

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinking with formaldehyde, sonication with bioruptor 16 cycles 30" on 30" off. ChIP with p53-DO1 antibody. DNA extracted with phenol/chloroform. ChIP-seq library was prepared according to manifacturer's suggestion using NEBNext ChIPSEq libraryQ5 Hot Start HiFi PCR Master Mix (NEB inc)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

52699131

Reads aligned (%)

87.6

Duplicates removed (%)

69.0

Number of peaks

5100 (qval < 1E-05)

hg19

Number of total reads

52699131

Reads aligned (%)

86.6

Duplicates removed (%)

70.5

Number of peaks

4714 (qval < 1E-05)