Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Epidermis

Cell type

Dermal fibroblast

NA

NA

Sample

source_name

foreskin HDF, Nutlin, INPUT

cell line

foreskin HDF

passage

p4

chip antibody

none

treatment

Nutlin-3a 10 μM

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinking with formaldehyde, sonication with bioruptor 16 cycles 30" on 30" off. ChIP with p53-DO1 antibody. DNA extracted with phenol/chloroform. ChIP-seq library was prepared according to manifacturer's suggestion using NEBNext ChIPSEq libraryQ5 Hot Start HiFi PCR Master Mix (NEB inc)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

133646644

Reads aligned (%)

91.5

Duplicates removed (%)

5.7

Number of peaks

3536 (qval < 1E-05)

hg19

Number of total reads

133646644

Reads aligned (%)

90.5

Duplicates removed (%)

7.3

Number of peaks

1882 (qval < 1E-05)