Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9ac

Cell type

Cell type Class
Spleen
Cell type
Leukemic spleen cells
NA
NA

Attributes by original data submitter

Sample

source_name
leukemic spleen cells
strain
Gfi1 36S/S-Nup98/HoxD13
tissue
spleen
chip-antibody
H3K9ac, Active Motif, Cat# 39917, Lot# 23913004

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
the spleens were isolated from NUP98/HOXD13 mice carrying either the human GFI136S or the human GFI136N cDNA. The spleens were homogenised and ery lysis was performed. 3x10^7 cells were fixed with 0.4% formaldehyde and sonicated for 20 min. Histone-DNA antibody were isolated with antibody Libraries were prepared using the NEBNext® UltraTM DNA Library Prep Kit for Illumina®. Samples were pooled after quality check.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
41024567
Reads aligned (%)
98.4
Duplicates removed (%)
22.8
Number of peaks
16261 (qval < 1E-05)

mm9

Number of total reads
41024567
Reads aligned (%)
98.2
Duplicates removed (%)
22.8
Number of peaks
16317 (qval < 1E-05)

Base call quality data from DBCLS SRA