Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PPARG

Cell type

Cell type Class
Digestive tract
Cell type
HT-29
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
PPARG_48h_Rosiglitazone
cell line
HT29
cell type
colorectal adenocarcinoma
agent
Rosiglitazone
time
48hr
chip antibody
anti-PPARG (Santa Cruz, sc-7273)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed as previously described (Reddy et al. Molecular Cell 2013). RNA-seq was performed as previously outlned (Gertz et al. 2012) Illumina Single End for ChIP-seq, Illumina Paired End for RNA-seq

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
31780844
Reads aligned (%)
65.9
Duplicates removed (%)
22.7
Number of peaks
17530 (qval < 1E-05)

hg38

Number of total reads
31780844
Reads aligned (%)
66.9
Duplicates removed (%)
21.9
Number of peaks
17562 (qval < 1E-05)

Base call quality data from DBCLS SRA