Antigen

Antigen Class

TFs and others

Antigen

PPARG

Cell type

Cell type Class

Digestive tract

Cell type

HT-29

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

PPARG_2h_Rosiglitazone

cell line

HT29

cell type

colorectal adenocarcinoma

agent

Rosiglitazone

time

2hr

chip antibody

anti-PPARG (Santa Cruz, sc-7273)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed as previously described (Reddy et al. Molecular Cell 2013). RNA-seq was performed as previously outlned (Gertz et al. 2012) Illumina Single End for ChIP-seq, Illumina Paired End for RNA-seq

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

19914283

Reads aligned (%)

96.2

Duplicates removed (%)

8.4

Number of peaks

28594 (qval < 1E-05)

hg19

Number of total reads

19914283

Reads aligned (%)

95.8

Duplicates removed (%)

9.0

Number of peaks

28649 (qval < 1E-05)