Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

perfused primary hepatocytes

strain

C57/B6 and 129 mix

tissue

liver

age

postnatal day 90

genotype

Arid1a Fl/Fl

chip antibody

NA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

liver were perfused and primary hepatocytes were isolated and performed for ChIP-seq. ChIP-seq libraries were prepared using KAPA HTP Library Preparation Kit, and sequenced on Illumina HiSeq 2500 using 50SR SBS v3 reagents at McDermott Center Next Generation Sequencing Core at UT Southwestern.And for H3K4me2 chip-seq, library were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina Kit, and sequenced on Illumina NEXTseq 500 using High Output kit v2 reagents at CRI Sequencing Facility at UT Southwestern.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

19767160

Reads aligned (%)

98.3

Duplicates removed (%)

28.8

Number of peaks

335 (qval < 1E-05)

mm9

Number of total reads

19767160

Reads aligned (%)

98.2

Duplicates removed (%)

29.0

Number of peaks

280 (qval < 1E-05)