Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

tissue

Haematopoietic and lymphoid

histology

Haematopoietic neoplasm

protein-dna complex

PolII

protocol

ChIP-exo

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-exo, lysates were clarified from sonicated nuclei and Protein(TFIIB, PolII)-DNA complexes were isolated with IgG antibody. PIP-Seq libraries were prepared for sequencing using standard Illumina protocols; Reference paper: http://www.ncbi.nlm.nih.gov/pubmed/23746353 ChIP-exo libraries were prepared for sequencing using standard Illumina protocols; Reference paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813302/

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

30195928

Reads aligned (%)

72.2

Duplicates removed (%)

44.6

Number of peaks

6936 (qval < 1E-05)

hg19

Number of total reads

30195928

Reads aligned (%)

71.4

Duplicates removed (%)

45.4

Number of peaks

6766 (qval < 1E-05)