Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

Preimplantation embryo

NA

NA

Sample

source_name

Preimplantation embryo

strain

C57BL/6N x PWK

tissue/cell type

Preimplantation embryo

chip antibody

H3K27Me3

chip antibody vendor

Diagenode

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. (PMID: 25223787) with slightly modification.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

16699620

Reads aligned (%)

66.1

Duplicates removed (%)

10.4

Number of peaks

67 (qval < 1E-05)

mm9

Number of total reads

16699620

Reads aligned (%)

66.0

Duplicates removed (%)

10.3

Number of peaks

60 (qval < 1E-05)