Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SON

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562 cells
cell line
K562
cell type
Chronic myelogenous leukemia
chip antibody
SON antibody recognizing the N-terminus of SON (amino acids 74-88 of human SON)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Gnomegen Library Preparation Kits (Gnomegen) and ChIP-seq libraries were cluster-amplified and sequenced with the Illumina HiSeq2000 sequencer (50-nucleotide pair-ended read) following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
26753051
Reads aligned (%)
136.3
Duplicates removed (%)
22.4
Number of peaks
750 (qval < 1E-05)

hg38

Number of total reads
26753051
Reads aligned (%)
137.5
Duplicates removed (%)
22.2
Number of peaks
799 (qval < 1E-05)

Base call quality data from DBCLS SRA