Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
PSU-m
lab description
Hardison - Penn State University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
age
immortalized
age description
Immortal cells
control
Input
control description
Control signal which may be subtracted from experimental raw signal before peaks are called.
controlid
wgEncodeEM001923
replicate
1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM946532: PSU_ChipSeq_CH12_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
213842248
Reads aligned (%)
90.3
Duplicates removed (%)
9.0
Number of peaks
1778 (qval < 1E-05)

mm9

Number of total reads
213842248
Reads aligned (%)
90.2
Duplicates removed (%)
9.0
Number of peaks
1939 (qval < 1E-05)

Base call quality data from DBCLS SRA