Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
SK-N-SH
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
Neuroblastoma
cell line
neuroblastoma cell line SK-N-SH
chip antibody
H3K4me3 (Millipore, catalog# 17-614)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and CTCF,Rad21,RNAPII, histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
12083221
Reads aligned (%)
99.5
Duplicates removed (%)
8.7
Number of peaks
23508 (qval < 1E-05)

hg19

Number of total reads
12083221
Reads aligned (%)
99.0
Duplicates removed (%)
9.1
Number of peaks
23541 (qval < 1E-05)

Base call quality data from DBCLS SRA