GSM2037802: shAFF1&4-HMBA; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 cells - shAFF1&4 - HMBA
cell type
Colon Cancer Cells
passages
10-15
cell line
HCT116
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The nuclei were incubated with Micrococcal Nuclease (MNase) (10 μg DNA per 1 unit MNase) to digest genomic DNA to the optimal length (1-2 nucleosomes), followed by sonication to break the nuclear membrane, with the presence of 0.1% SDS. ChIP was performed with anti-Rpb1(Pol II) antibody. Libraries were constructed by RiboBio Co. Ltd. (Guangzhou,China).