Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Lung
Cell type
NCI-H1299
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Non-Small Cell

Attributes by original data submitter

Sample

source_name
HIF-1alpha, H1299
cell line
H1299
chip antibody
HIF-1alpha

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. genomic DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
12227639
Reads aligned (%)
70.6
Duplicates removed (%)
23.9
Number of peaks
538 (qval < 1E-05)

hg38

Number of total reads
12227639
Reads aligned (%)
75.4
Duplicates removed (%)
20.2
Number of peaks
136 (qval < 1E-05)

Base call quality data from DBCLS SRA