Antigen

Antigen Class

Histone

Antigen

H3K79me2

Cell type

Cell type Class

Blood

Cell type

Common myeloid progenitor

NA

NA

Sample

source_name

myeloid leukemia

cell type

Bone marrow leukemic blasts sorted from moribund mice that had been transplanted with CMP-cells transformed with MN1

genotype

Genotype: Dot1l f/f (conditional allele, NOT inactivated)

strain

C57BL/6

chip-antibody

Abcam anti-H3K79me2 (ab3594)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was isolated using standard ChIP protocol Nugen Ovation Ultra low system V2

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

33621887

Reads aligned (%)

99.0

Duplicates removed (%)

17.5

Number of peaks

6755 (qval < 1E-05)

mm9

Number of total reads

33621887

Reads aligned (%)

98.9

Duplicates removed (%)

17.6

Number of peaks

6757 (qval < 1E-05)