Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
UBTF

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
H9 hESC treated with a 50 ng/mL final concentration of Activin A for 6 hours
cell line
H9
chip antibody
anti-UBTF (Millipore # MABE195)
passage
58-60
treatment
50 ng/mL Activin A for 6 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linked nuclei were sonicated and UBTF was purified using indicated antibody and protein A beads. Immunopurified DNA was reverse-cross-linked, phenol:chloroform extracted and ethanol precipitated. Library was constructed using Illuina Tru-seq branched adapters and subjected to 25 PCR cycles

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
17485609
Reads aligned (%)
60.2
Duplicates removed (%)
19.5
Number of peaks
371 (qval < 1E-05)

hg38

Number of total reads
17485609
Reads aligned (%)
60.8
Duplicates removed (%)
19.2
Number of peaks
298 (qval < 1E-05)

Base call quality data from DBCLS SRA