GSM2028275: ChIP-seq UBTF 6hr ActivinA; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
UBTF
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
H9 hESC treated with a 50 ng/mL final concentration of Activin A for 6 hours
cell line
H9
chip antibody
anti-UBTF (Millipore # MABE195)
passage
58-60
treatment
50 ng/mL Activin A for 6 hours
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linked nuclei were sonicated and UBTF was purified using indicated antibody and protein A beads. Immunopurified DNA was reverse-cross-linked, phenol:chloroform extracted and ethanol precipitated. Library was constructed using Illuina Tru-seq branched adapters and subjected to 25 PCR cycles