Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H2Bub

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
embryonic stem cells
cell type
mouse embryonic stem cell
cell line
J1
chip antibody
H2Bub1(Millipore, 17-650)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution in J1 mouse ES cells. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for the generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
26779826
Reads aligned (%)
71.0
Duplicates removed (%)
23.8
Number of peaks
436 (qval < 1E-05)

mm9

Number of total reads
26779826
Reads aligned (%)
70.9
Duplicates removed (%)
23.9
Number of peaks
415 (qval < 1E-05)

Base call quality data from DBCLS SRA