Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Neural

Cell type

SH-SY5Y

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

SH-SY5Y

cell type

EPOP Overexpression

chip antibody

H3K4me3; Millipore, #04-745

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA were extracted using antibodies for EPOP, H3K4me3, Elongin B, RNA Polymerase II S5, USP7 or H2Bub. RNA-Seq samples were prepared by isolating total RNA using Trizol, mRNA Isolation, heat fractionation, reverse transcription and second strand synthesis Libraries were constructed of 10-50 ng of DNA following Illumina's® protocol

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

26540827

Reads aligned (%)

97.1

Duplicates removed (%)

32.8

Number of peaks

22457 (qval < 1E-05)

hg19

Number of total reads

26540827

Reads aligned (%)

97.0

Duplicates removed (%)

32.9

Number of peaks

22598 (qval < 1E-05)