Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Kasumi-1_ChIP-seq
cell line
Kasumi-1
cell type
human acute myeloid leukemia cell line
antibody target
RUNX1
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sonicated and used for ChIP seq using specific antibodies (avialable on suplementry table). RNA was extracted using qiagen rneasy and treated with ribozero kit. ChIP seq libraries were preapared according to Illumina's standard protocol. For RNA-seq cDNA was prepared according to strand specific and libraries were preapared according to Illumina's standard protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
31690343
Reads aligned (%)
48.9
Duplicates removed (%)
49.7
Number of peaks
22797 (qval < 1E-05)

hg38

Number of total reads
31690343
Reads aligned (%)
49.7
Duplicates removed (%)
49.0
Number of peaks
22860 (qval < 1E-05)

Base call quality data from DBCLS SRA