Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

WCE_p20

cell type

p20 iPS whole cell extract

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed as described previously (van Oevelen et al., 2008) using an antibody against H3K27ac (ab4729, Abcam) and CTCF (Millipore, 07-729). DNA libraries were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

38172765

Reads aligned (%)

97.8

Duplicates removed (%)

18.2

Number of peaks

505 (qval < 1E-05)

mm9

Number of total reads

38172765

Reads aligned (%)

97.5

Duplicates removed (%)

18.1

Number of peaks

563 (qval < 1E-05)