Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
WCE_p3
cell type
p3 iPS whole cell extract

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed as described previously (van Oevelen et al., 2008) using an antibody against H3K27ac (ab4729, Abcam) and CTCF (Millipore, 07-729). DNA libraries were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
27641869
Reads aligned (%)
97.9
Duplicates removed (%)
14.9
Number of peaks
407 (qval < 1E-05)

mm9

Number of total reads
27641869
Reads aligned (%)
97.7
Duplicates removed (%)
14.9
Number of peaks
440 (qval < 1E-05)

Base call quality data from DBCLS SRA