Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Bone Marrow Cells
MeSH Description
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Attributes by original data submitter

Sample

source_name
Bone Marrow
tissue
Bone Marrow
genotype/variation
Normal
strain
Klf4flox/flox vav-Cre in C57BL/6 background.
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sorted cells were fixed and then genomic DNA was extracted and sheared. DNA was reverse crosslinked after KLF4 antibody pulldown and was subjected to seuqencing. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on HiSeq 2500.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
42970025
Reads aligned (%)
99.2
Duplicates removed (%)
10.3
Number of peaks
629 (qval < 1E-05)

mm9

Number of total reads
42970025
Reads aligned (%)
99.0
Duplicates removed (%)
10.3
Number of peaks
629 (qval < 1E-05)

Base call quality data from DBCLS SRA