Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF143

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 cells
cell line
MCF-7
chip-antibody
ZNF143 (Novus Biologicals H00007702-M01)
treatment
10 uM 17-beta oestradiol (E2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sonicated by biorupter and immunoprecipitated with anti-ZNF143 antibody. DNA was extracted using MinElute PCR purification kit (Qiagen 28004). Library construction was performed using New England Biolabs ChIP-seq library prep reagent set (E6200S)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
62181308
Reads aligned (%)
12.0
Duplicates removed (%)
87.7
Number of peaks
11281 (qval < 1E-05)

hg38

Number of total reads
62181308
Reads aligned (%)
12.5
Duplicates removed (%)
87.2
Number of peaks
11340 (qval < 1E-05)

Base call quality data from DBCLS SRA